4DN consortium recommended cell lines

  • Tier 1: small set of cells provided to each 4DN member to be able to use and provide data for all studies.
  • Tier 2: large set of cells based on popular usage for specific projects.
By clicking on a cell line name you can access more information from the 4DN Data Portal, including SOPs.


Tier 1 Description Provider Availability
H1-ESC (WA01) Human embryonic stem cells (male) WiCell
GM12878 EBV-Immortalized B-lymphocytes (female) Coriell
IMR90 Human Fetal Lung Fibroblast ATCC
HFF-hTert-clone 6 hTert-immortalized human foreskin fibroblasts (male) Job Dekker Lab
WTC-11 Induced pluripotent stem cells (iPSc male) Bruce Conklin Lab
Tier 2 Description Provider Availability
F121-9 Mouse Cast-129 F1 hybrid embryonic stem cells (female) David Gilbert Lab
F123 Mouse Cast-129 F1 hybrid embryonic stem cells (male) Bing Ren Lab
HAP1 Haploid derivative of chronic myelogenous leukemia Bas van Steensel Lab
H9 Human embryonic stem cells (female) Wicell
hTERT-RPE hTERT immortalized retina pigmented epithelium (female) ATCC
K562 Chronic myelogenous leukemia (female) ATCC
HEK293 Human embryonic kidney (female) ATCC
U2OS Osteosarcoma cells (female) ATCC
JM8.N4 Mouse Embryonic Stem Cells Komp
HCT 116 Human Colorectal carcinoma ATCC
G4 Mouse Embryonic Stem Cells Carol Ware

Eligibility information for PIs who are new to 4DN Cell Repository

All current Phase II PIs have been approved by the Phase II 4DN Cells WG to receive a single vial of each of our commercial repository stocked Tier1 cell lines, including: H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC). Additional vials will require separate approval from the Cells WG.

NOTE: All PIs are reminded to stock the cells by 4DN standards and to follow the 4DN approved SOP for all cell lines. When data will be submitted to the DCIC, they will need to verify that the cells have been expanded in these conditions that are posted on the Cells WG Wiki page, and the appropriate metadata will need to be recorded at the time of cell harvest in order for datasets to receive the gold standard flag.